merck millipore,默克密理博,MABE254,Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	ChIP, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABE254
描述	Anti-Protein-arginine deiminase 4 Antibody, clone 10F1
Alternate Names	Protein-arginine deiminase type IV HL-60 PAD Protein-arginine deiminase type-4 Peptidylarginine deiminase IV
背景信息	Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.

产品信息
格式	Purified
控制	DMSO treated and untreated HL-60 cell lysates
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein-arginine deminiase 4 in IP (Prof. K. Mowen, Department of Chemical Physiology, The Scripps Research Institute-California Campus.). Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Protein-arginine deminiase 4 in ChIP-Seq (Price, D. H., et al. (2011). Mol Cell. 45(1):38-50.).

生物信息
免疫原品种	GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.
克隆	10F1
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG1κ
品种反应性	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_036519
基因符号	PDI5 PADI4 PADI5
纯化方法	Protein G purified
UniProt编号	Q9UM07

产品使用声明
质量保证	Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates. Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MABE254,Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

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