merck millipore,默克密理博,MABE15,Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

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merck millipore,默克密理博,MABE15,Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal
产品名称：Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal
产品型号：MABE15
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,MABE15,Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE15,Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABE15
描述	Anti-Histone H2B Antibody, clone EP957Y, rabbit monoclonal
Alternate Names	histone H2B type 2-E histone cluster 2, H2be histone 2, H2be H2B histone family, member Q Histone H2B.q Histone H2B-GL105
背景信息	Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

产品信息
格式	Unpurified
控制	A431 cell lysate
演示	Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:250 – 1:500 dilution from a previous lot detected Histone H2B in human breast carcinoma. Immunoprecipitation Analysis: A 1:30 dilution from a previous lot detected Histone H2B in IP. Immunocytochemistry Analysis: A 1:50 – 1:100 dilution from a previous lot detected Histone H2B in HeLa cells.

生物信息
免疫原品种	Synthetic peptide corresponding to human Histone H2B.
克隆	EP957Y
宿主	Rabbit
特异性	This antibody recognizes Histone H2B.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001019770.1
基因符号	MGC119802 H2B.1 H2B/q H2BFQ H2B MGC119804 MGC129733 H2BGL105 GL105 H2BQ MGC129734
纯化方法	Unpurified
UniProt编号	Q16778
UniProt汇总	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. Chromosome. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. SEQUENCE SIMILARITIES: Belongs to the histone H2B family.

产品使用声明
质量保证	Evaluated by Western Blot in A431 cell lysate. Western Blot Analysis: A 1:1,000 - 1:10,000 dilution of this antibody detected Histone H2B in A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.