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merck millipore,默克密理博,MABD172,Anti-THAP11 Antibody, clone 3F3
产品名称：Anti-THAP11 Antibody, clone 3F3
产品型号：MABD172
Anti-THAP11 Antibody, clone 3F3 is a highly specific mouse monoclonal antibody, that targets Thanatos-associated protein 11 & has been tested in western blotting, Immunofluorescence, IHC & Flow Cytome More>>
merck millipore,默克密理博,MABD172,Anti-THAP11 Antibody, clone 3F3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABD172 描述 Anti-THAP11 Antibody, clone 3F3 Alternate Names - THAP domain-containing protein 11
背景信息 THAP11 or Thanatos-associated protein 11, THAP domain-containing protein 11, encoded by the human gene named THAP11 or HRIHFB2206 is a sequence specific transcription factor critical for embryogenesis and the pluripotency of embryonic stem cells (ESCs). THAP11 is a transcriptional repressor that suppresses differentiation genes in ES cells by suppressing c-Myc. Overexpression of THAP11 markedly inhibits growth of a number of different cells, including some cancer cells and non-transformed cells. THAP11 is ubiquitously expressed in normal tissues and frequently downregulated in several human tumor tissues. Research demonstrates that among the cellular protein partners of THAP11 is a transcriptional activator PCBP1, where again overexpression inhibited CD44v6 expression (one of the homing receptors) and cell invasion in the cancer cell line HepG2. However THAP11 is not always associated with growth suppression as recent research in colon cancer indicates that THAP11 associates with the transcriptional coregulator HCF-1 (host cell factor 1) and recruits HCF-1 to target promoters leading to an increase in gene expression. Moreover THAP11 is found to be expressed in both primary tumors and metastases, and knockdown of THAP11 in SW620 colon cancer cells lines showed a marked decrease in cell proliferation and a down regulation of numerous genes by gene profiling. EMD-Millipore’s Anti-THAP11 mouse monoclonal antibody has been tested in western blot against HeLa and NTERA-2 cell lysates and paraffin embedded immunohistochemistry on colon and ovarian cancer tissue sections and in fluorescent immunocytochemistry on NTERA-2 cells in culture and in flow cytometry on HeLa cells in culture. 产品信息 格式 Ascites 控制 - HeLa and NTERA-2 cell lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Anti-THAP11 Antibody, clone 3F3 is a highly specific mouse monoclonal antibody, that targets Thanatos-associated protein 11 & has been tested in western blotting, Immunofluorescence, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected THAP11 in NTERA-2 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected THAP11 in paraffin-embedded colon cancer and ovarian tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected THAP11 in HeLa cells.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human THAP11 expressed in E. Coli. 克隆 3F3 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 基因符号 - THAP11
- RONIN
- CTG-B43a
- CTG-B45d
- HRIHFB2206
纯化方法 Unpurified UniProt编号 - Q96EK4
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa and NTERA-2 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected THAP11 in HeLa and NTERA-2 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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