- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABC87,Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)
产品名称：Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)
产品型号：MABC87
This Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Inhibition and Immunoprecipitation for the detection of CD148/DE More>>
merck millipore,默克密理博,MABC87,Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 MABC87 描述 Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) Alternate Names - R-PTP-J
- CD148
- DEP-1
- Receptor-type tyrosine-protein phosphatase eta
- Protein-tyrosine phosphatase eta
- R-PTP-eta
- Density-enhanced phosphatase 1
- DEP1
- HPTP eta
- Protein-tyrosine phosphatase receptor type J
背景信息 CD148, also known as Density-enhanced phosphatase 1 (DEP-1), is a membrane bound phosphatase that dephosphorylates a large number of protein targets and kinases thus serving as a regulator of many signaling cascades. CD148/DEP-1 plays a role in cell adhesion, migration, proliferation and differentiation. CD148/DEP-1 also plays a role in macrophage migration, adhesion and spreading. CD148/DEP-1 is also central to T-cell receptor signaling and acts as a negative regulator. CD148/DEP-1 acts as a positive regulator of endothelial cell survival and enhances the barrier function of epithelial junctions during reassembly. CD148/DEP-1 is also a key regulator of platelet activation and thrombosis as well as a tumor suppressor. CD148/DEP-1 is localized at the cell membrane particularly at cell projections, ruffle membranes, and cell junctions and it is expressed in lymphocytes and epithelial cells and expression increases as cell density increases. 产品信息 格式 Purified 演示 Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives. 应用 应用 This Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Inhibition and Immunoprecipitation for the detection of CD148/DEP-1. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Raji, HEK293 +/-CD148 HA transfection, and A431D +/- CD148 transfection cell lysates.
Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Mouse kidney lysates (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected CD148/DEP-1 in Epithelial Cells of human prostate tissue.
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in frozen mouse embryos. (Takahashi, T., et al., (2003) MOLECULAR AND CELLULAR BIOLOGY. 23(5):1817–1831).
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in various mouse tissues such as kidney, cortex and cornea. (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Inhibition Assay: A representative lot of this antibody inhibited proliferation of HRMEC's (Primary human renal microvascular endothelial cells) (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated CD148/DEP-1 from Huvec and RMEC cell lysates (Takahashi, T., et al., (1999) J. Am. Soc. Nephrol. 10: 2135–2145).生物信息 免疫原品种 His-tagged recombinant protein corresponding to Human CD148/DEP-1. 克隆 Ab 1 浓缩 Please refer to lot specific datasheet. 宿主 Mouse 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Human, Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001091973
基因符号 - DEP1
- PTPRJ
纯化方法 Protein A purified UniProt编号 - Q12913
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD148/DEP-1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,07-625,Anti-ATRIP Antibody
下一件merck millipore产品：merck millipore,默克密理博,SF-166,miR-194-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare
相关产品
merck millipore,默克密理博,07-595,Anti-acetyl-Histone H4 (Lys12) Antibody
merck millipore,默克密理博,SF-2439,CSF1R; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,07-303,Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody
merck millipore,默克密理博,04-1124,Anti-SIRT2 (CT) Antibody, clone EP1668Y, rabbit monoclonal
merck millipore,默克密理博,07-625,Anti-ATRIP Antibody
merck millipore,默克密理博,SF-166,miR-194-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MAB1276,Anti-Nuclei & Chromosomes Antibody, histone H1 protein, clone 1415-1
merck millipore,默克密理博,AB9210,Anti-Insulin Degrading Enzyme Antibody
merck millipore,默克密理博,05-806,Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
merck millipore,默克密理博,PSRP010R5,Millicell-24 插入式细胞培养板, PET, 1.0 µm