- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABC83 描述 Anti-Vsp4/SKD1 Antibody, clone 5E6.1 Alternate Names - Protein SKD1
- Vacuolar protein sorting-associated protein 4B
- Cell migration-inducing gene 1 protein
- Suppressor of K(+) transport growth defect 1
背景信息 Vacuolar protein sorting-associated protein 4B (Vps4), or SKD1, is a ubiquitous AAA ATPase involved in endosomal vesicle trafficking. Trafficking of vesicles containing proteins such as receptors, from the endosome to the cytoplasm, is an important regulatory mechanism for cell-surface receptors and the signaling pathways that involve those receptors. Such endosomal vesicles are shuttled from the endosome to the cytoplasm by multivesicular bodies (MVB). MVBs are large multi-protein complexes that assemble and disassemble during this process. Previous studies have indicated that the yeast homologue of Vps4, Vps4p (60 % sequence identity to Vps4), interacts with endosomes via its N-terminal microtubule interacting and trafficking (MIT) domain, and also associates with a number of MVB proteins including Vps2p, Bro1p, Vps20p, Vta1p, and Did2p/Chm1p. Although the precise role of Vps4 is still unclear, previous studies have suggested that it interacts with the ESCRT-III complex involved in the sorting of MVBs and has been associated with virus-loaded vesicles such those in HIV-infected cells. 产品信息 格式 Purified 控制 - Human skeletal muscle tissue lysate.
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Vsp4/SKD1 Antibody, clone 5E6.1 is an antibody against Vsp4/SKD1 for use in Western Blotting, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Vsp4/SKD1 in NIH/3T3, A431, and HeLa cells. 生物信息 免疫原品种 Linear peptide corresponding to human Vps4/SKD1. 克隆 5E6.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_004860
Entrez基因汇总 The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. 基因符号 - MIG1
- VPS4B
- SKD1
- VPS42
纯化方法 Protein G purified UniProt编号 - O75351
UniProt汇总 FUNCTION: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
SUBUNIT STRUCTURE: Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. In vitro, associates on the inside of a helical tubular structure formed by a CHMP2A-CHMP3 polymer. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP4B and CHMP6. Interacts with VPS4A; the interaction suggests a heteromeric assembly with VPS4A. Interacts with VTA1.
SUBCELLULAR LOCATION: Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein probable. Note: Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
TISSUE SPECIFICITY: Ubiquitously expressed.
DOMAIN: The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
POST-TRANSLATIONAL MODIFICATION: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the AAA ATPase family. Contains 1 MIT domain.产品使用声明 质量保证 Evaluated by Western Blot in human skeletal muscle tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Vsp4/SKD1 in 10 µg of human skeletal muscle tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,05-561,Anti-LAT Antibody, clone 2E9
下一件merck millipore产品：merck millipore,默克密理博,SF-1414,OLIG2; Mouse, Cyanine 5 RNA Detection Probe,SmartFlare
相关产品
merck millipore,默克密理博,14-671M,STK33 Protein, active, 250 µg
merck millipore,默克密理博,480354,7S NGF, Mouse Submaxillary Glands
merck millipore,默克密理博,ABE481,Anti-Centrin-3 Antibody
merck millipore,默克密理博,ABN425,Anti-DISC1 Antibody
merck millipore,默克密理博,05-561,Anti-LAT Antibody, clone 2E9
merck millipore,默克密理博,SF-1414,OLIG2; Mouse, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,FCMAB313PE,Milli-Mark Anti-Nestin-PE Antibody, clone 10C2
merck millipore,默克密理博,EZHCMRN-57K,Human Chemerin ELISA
merck millipore,默克密理博,SF-738,CTNNB1; Human, Cyanine 3, RNA Detection Probe,SmartFlare