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merck millipore,默克密理博,MAB5520,Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1
产品名称：Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1
产品型号：MAB5520
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in FC & WB.
merck millipore,默克密理博,MAB5520,Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB5520 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 Alternate Names - NG2
产品信息 格式 Ascites 演示 Ascites fluid. Liquid. Contains no preservative. 应用 应用 Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1 is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in FC & WB. 主要应用 - Western Blotting
- Flow Cytometry
应用说明 Western blot
Flow cytometry
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human bone marrow stromal cell line, SV40 transformed. 克隆 7.1 宿主 Mouse 特异性 NG2 (chondroitin sulfate proteoglycan), human which is the homologue of the rat NG2 molecule. The antibody reacts with a 220-240 kD surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule.
MAB5520 reacts with only neoplastic leukocytes with specific association with chromosome 11q23 translocations involving the MLL gene. It has a very high specificity for t(4;11) or t(11;19) translocations. The antibody shows no reactivity with normal marrow hematopoietic cells, peripheral blld leukocytes, red blood cells or platelets. Leukemic cells lines KG1, KG1a, HRL, Jurkat, K562, Raji, HL-60, U937, Daudi, Nalm6 and CEM are negative. However MAB5520 does react with a variety of cell lines of nonhematopoietic origin, including HeLa cells.同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001897.4
Entrez基因汇总 A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. 基因符号 - HMW-MAA
- CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
UniProt编号 - Q6UVK1
UniProt汇总 FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
SIZE: 2322 amino acids; 250497 Da
SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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