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品种反应性 主要应用 宿主 格式 抗体类型 Ch WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB5234 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurocan Antibody 背景信息 Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration. 产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in PBS. Contains no preservative. 应用 应用 Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: < 0.5 μg/mL
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Mixture of chicken chondroitin sulfate proteoglycans. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Neurocan, chicken. Recognizes the middle region of neurocan. 同种型 IgG1 品种反应性 Chicken 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004386.2
Entrez基因汇总 Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM] 基因符号 - CSPG3
- NCAN
- FLJ44681
- NEUR
UniProt编号 - O14594
UniProt汇总 FUNCTION: SwissProt: O14594 # May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
SIZE: 1321 amino acids; 143093 Da
TISSUE SPECIFICITY: Brain.
SIMILARITY: SwissProt: O14594 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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