merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB5212,Anti-Neurocan Antibody, clone 650.24

  • merck millipore,默克密理博,MAB5212,Anti-Neurocan Antibody, clone 650.24

    产品名称：Anti-Neurocan Antibody, clone 650.24
    产品型号：MAB5212
    This Anti-Neurocan Antibody, clone 650.24 is validated for use in IP, WB, IC, IH for the detection of Neurocan.

    merck millipore,默克密理博,MAB5212,Anti-Neurocan Antibody, clone 650.24

  • 产品介绍
  • merck millipore,默克密理博,MAB5212,Anti-Neurocan Antibody, clone 650.24

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    RIHC, WB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB5212
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Neurocan Antibody, clone 650.24
    Alternate Names
    • 245 kDa Early Postnatal Core Glycoprotein
    背景信息 Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.
    产品信息
    格式 Purified
    控制
    • POSITIVE CONTROL:

      early post-natal rat brain. Not expressed in kidney, lung, liver, or muscle.
    演示 Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
    应用
    应用 This Anti-Neurocan Antibody, clone 650.24 is validated for use in IP, WB, IC, IH for the detection of Neurocan.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Immunocytochemistry
    应用说明 Western blot: 1-2 μg/mL. Reacts with polypeptides of 260 and 160 kDa on western blots of embryonic rat brain tissue extracts. The 160 kDa species is typically only seen aftyer chondroitinase treatment.Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

    Immunocytochemistry: 1:5

    Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

    Immunoprecipitation: 1-2 μg/mL

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Embryonic rat brain proteoglycans
    克隆 650.24
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Neurocan. The specificity of clone 650.24 was checked by western blots of brain lysates and purified neurocan; the antibody reacted with the identical bands that clones 1D1 and 1F6 (other neurocan monoclonals) reacted; furthermore, the staining pattern of brain sections with these antibodies were all identical. Finally 650.24 also recognized recombinant neurocan expressed in transfected 293 cells by western blot.
    同种型 IgG1
    品种反应性 Rat
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_004386.2
    Entrez基因汇总 Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM]
    基因符号
    • CSPG3
    • NCAN
    • FLJ44681
    • NEUR
    UniProt编号
    • O14594
    UniProt汇总 FUNCTION: SwissProt: O14594 # May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
    SIZE: 1321 amino acids; 143093 Da
    TISSUE SPECIFICITY: Brain.
    SIMILARITY: SwissProt: O14594 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB5212,Anti-Neurocan Antibody, clone 650.24

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