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品种反应性 主要应用 宿主 格式 抗体类型 H ELISA, WB, FC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4401 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Oct-4 Antibody, clone 10H11.2 概述 Purified monoclonal antibody for the detection of Human Oct-4. Alternate Names - octamer-binding transcription factor-3
- POU-type homeodomain-containing DNA-binding protein
- POU domain, class 5, transcription factor 1
- POU domain class 5, transcription factor 1
- POU class 5 homeobox 1
- Octamer-binding transcription factor 3
- POU class 5 homeobox 1
- Octamer-binding transcription factor 3
背景信息 Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. 产品信息 格式 Purified 控制 - Human embryonic stem cell lysate.
演示 Purified Ascites presented as a liquid in PBS with 0.1% sodium azide. 应用 应用 Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA. 主要应用 - ELISA
- Western Blotting
- Flow Cytometry
- Immunocytochemistry
应用说明 Flow Cytometry: A previous lot of this antibody was used in FC.
ELISA: A previous lot of this antibody was used in ELISA.
Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant GST fusion protein, human Oct-4 (aa 1-134). 克隆 clone 10H11.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells). 同种型 IgG1 品种反应性 Human Species Reactivity Note The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002701.4
基因符号 - Oct-4
- POU5F1
- Oct-3
- MGC22487
- Oct4
- OTF4
- OTF3
- OCT3
- OCT4
纯化方法 Protein A purfied UniProt编号 - Q01860
UniProt汇总 FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
SIZE: 360 amino acids; 38571 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.产品使用声明 质量保证 Evaluated by western blot on mouse P1 brain lysates.
Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at from date of receipt. 包装信息 数量 100 µg
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