merck millipore,默克密理博,235419,Caspase-3 Cellular Activity Assay Kit

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merck millipore,默克密理博,235419,Caspase-3 Cellular Activity Assay Kit
产品名称：Caspase-3 Cellular Activity Assay Kit
产品型号：235419

merck millipore,默克密理博,235419,Caspase-3 Cellular Activity Assay Kit

产品介绍

merck millipore,默克密理博,235419,Caspase-3 Cellular Activity Assay Kit

重要规格表

检测方法
Fluorometric or colorimetric

描述
概述	A convenient assay kit useful for measuring the caspase-3 and caspase-3-like activities in cell extracts using either a colorimetric or fluorometric substrate. Provided in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. In addition, caspase-3 is included for use as a positive control, for testing cell extracts for endogenous inhibitors, or for comparing effects of exogenous inhibitors on cellular activity versus the activity of the purified enzyme.
产品目录编号	235419
品牌系列	Calbiochem®

参考
参考	Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Faleiro, L., et al. 1997. EMBO J. 16, 2271. Kothakota, S., et al. 1997. Science 278, 294. Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297. Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486. Nicholson, D.W., et al. 1995. Nature 376, 37. Tewari, M., et al. 1995. Cell 81, 801. Fernandes-Alnemi, T., et al. 1994. J.Biol. Chem. 269, 30761.

参考

Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Faleiro, L., et al. 1997. EMBO J. 16, 2271.
Kothakota, S., et al. 1997. Science 278, 294.
Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Fernandes-Alnemi, T., et al. 1994. J.Biol. Chem. 269, 30761.

产品信息
检测方法	Fluorometric or colorimetric
形式	96 Tests
格式	96-well plate
试剂盒包含	Human Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.

生物信息
化验时间	2.5-3 h
样品类型	Cell extracts
品种	a broad range of species

安全信息
R相	R: 23/24/25-63-36/37/38-33 Toxic by inhalation, in contact with skin and if swallowed. Possible risk of harm to the unborn child. Irritating to eyes, respiratory system and skin. Danger of cumulative effects.
S相	S: 36/37/39-45-26-23 Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Do not breathe fumes.

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	≤ -70°C
存储条件	Upon arrival store the 1/2 volume plate at room temperature and the remaining components of the kit at -70°C.
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

补充信息
试剂盒包含	Human Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.