merck millipore,默克密理博,MAB4349,Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Fe, H, Po, Rb	ICC, FC	M	Alkaline Phosphatase	Monoclonal Antibody

描述
产品目录编号	MAB4349
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
背景信息	In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

产品信息
格式	Alkaline Phosphatase
控制	Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
演示	Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide

应用
应用	This Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E is validated for use in FC, IF, IC, IP for the detection of TRA-2-49.
主要应用	Immunocytochemistry Flow Cytometry
应用说明	Immunocytochemistry: A previous lot was used on IC. Immunofluorescence: A previous lot of this antibody was used in IF. FACS Analysis: A starting range of 5-20 µg/mL is suggested. Immunoprecipitation: A previous lot of this antibody was used in IP. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	2102Ep human Embryonal Carcinoma Cells
表位	Liver/Bone/Kidney Alkaline Phosphatase
克隆	clone TRA-2-49/6E
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.
同种型	IgG1
品种反应性	FelineHumanPigRabbit
Species Reactivity Note	Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.
抗体类型	Monoclonal Antibody
基因符号	TRA-2-49
非反应性品种	Rat Guinea Pig Monkey Mouse
纯化方法	Protein A purfied

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MAB4349,Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

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