merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB4334B,Anti-MDR1 Antibody, clone UIC2, biotin conjugated

  • merck millipore,默克密理博,MAB4334B,Anti-MDR1 Antibody, clone UIC2, biotin conjugated

    产品名称：Anti-MDR1 Antibody, clone UIC2, biotin conjugated
    产品型号：MAB4334B
    Anti-MDR1 Antibody, clone UIC2, biotin conjugated detects level of MDR1 & has been published & validated for use in FC, IP, IH, IH(P).

    merck millipore,默克密理博,MAB4334B,Anti-MDR1 Antibody, clone UIC2, biotin conjugated

  • 产品介绍
  • merck millipore,默克密理博,MAB4334B,Anti-MDR1 Antibody, clone UIC2, biotin conjugated

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Pm, HIHC, FCMBiotinMonoclonal Antibody
    描述
    产品目录编号 MAB4334B
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MDR1 Antibody, clone UIC2, biotin conjugated
    Alternate Names
    • Pgp
    • P-glycoprotein
    • CD243
    • p170
    背景信息 UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (Chemicon's UIC2 Shift assay - see product ECM905).
    产品信息
    格式 Biotin
    演示 Sterile filtered biotin conjugated immunoglobulin in 0.01M PBS, 0.1% Sodium Azide, 15mg/mL BSA. Biotin Conjugated Purified immunoglobulin
    应用
    应用 Anti-MDR1 Antibody, clone UIC2, biotin conjugated detects level of MDR1 & has been published & validated for use in FC, IP, IH, IH(P).
    主要应用
    • Immunohistochemistry
    • Flow Cytometry
    应用说明 Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.

    Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.

    Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.

    Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.

    IHC on frozen and paraffin sections: 1 - 10 μg/mL.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
    表位 conformational extracellular epitope
    克隆 UIC2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
    同种型 IgG2aκ
    品种反应性 PrimateHuman
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000927.3
    Entrez基因汇总 The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
    基因符号
    • EC 3.6.3.44
    • ABCB1
    • PGY1
    • MGC163296
    • CD243
    • GP170
    • P-gp
    • MDR1
    • CLCS
    • ABC20
    非反应性品种
    • Rat
    • Mouse
    UniProt编号
    • P08183
    UniProt汇总 FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    SIZE: 1280 amino acids; 141463 Da
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
    SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C for up 12 months from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB4334B,Anti-MDR1 Antibody, clone UIC2, biotin conjugated

上一件merck millipore产品：merck millipore,默克密理博,MABE345,Anti-methylcytosine Antibody, clone 87G31
下一件merck millipore产品：merck millipore,默克密理博,14-802,IGF-1R Protein, activated, 10 µg


相关产品

merck millipore,默克密理博,S8004,CpGenome™ 5-mC & 5-hmC Mouse DNA Standards merck millipore,默克密理博,S8004,CpGenome™ 5-mC & 5-hmC Mouse DNA Standards

merck millipore,默克密理博,SF-424,miR-29a-3p; Human, Cyanine 5, RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-424,miR-29a-3p; Human, Cyanine 5, RNA Detection Probe,SmartFlare

merck millipore,默克密理博,MACR08127,用于 7 mL 瓶的 MultiScreen 打孔器 merck millipore,默克密理博,MACR08127,用于 7 mL 瓶的 MultiScreen 打孔器

merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

merck millipore,默克密理博,MABE345,Anti-methylcytosine Antibody, clone 87G31 merck millipore,默克密理博,MABE345,Anti-methylcytosine Antibody, clone 87G31

merck millipore,默克密理博,14-802,IGF-1R Protein, activated, 10 µg merck millipore,默克密理博,14-802,IGF-1R Protein, activated, 10 µg

merck millipore,默克密理博,ABC274,Anti-QSOX1 Antibody merck millipore,默克密理博,ABC274,Anti-QSOX1 Antibody

merck millipore,默克密理博,NE1024,Anti-p75 Neurotrophin Receptor Rabbit pAb merck millipore,默克密理博,NE1024,Anti-p75 Neurotrophin Receptor Rabbit pAb

merck millipore,默克密理博,CBL468P,Anti-PECAM-1 Antibody, clone HC1/6, Phycoerythrin conjugated merck millipore,默克密理博,CBL468P,Anti-PECAM-1 Antibody, clone HC1/6, Phycoerythrin conjugated

merck millipore,默克密理博,SF-457,miR-30c-5p; Human, Cyanine 5, RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-457,miR-30c-5p; Human, Cyanine 5, RNA Detection Probe,SmartFlare


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部