merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

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merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal
产品名称：Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal
产品型号：04-1153
Use Anti-C/EBP β (C-term) Antibody, clone E299 (Rabbit Monoclonal Antibody) validated in WB, ICC, IP to detect C/EBP β (C-term) also known as CCAAT/enhancer binding protein beta.

merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1153,Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1153
Replaces	04-1099
描述	Anti-C/EBP β (CT) Antibody, clone E299, rabbit monoclonal
Alternate Names	nuclear factor of interleukin 6 C/EBP beta CCAAT/enhancer binding protein (C/EBP), beta CCAAT/enhancer binding protein beta Nuclear factor NF-IL6 Transcription factor 5 interleukin 6-dependent DNA-binding protein liver-enriched transcriptional activator protein
背景信息	The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPβ regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPβ is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32 kDa in rodents, ~43 kDa and 40 kDa in humans) and a truncated inhibitory form known as LIP (~20 kDa) which lacks the N-terminal transcription activation domain. C/EBP β is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPβ is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.

产品信息
格式	Unpurified
控制	PC-12 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Use Anti-C/EBP β (C-term) Antibody, clone E299 (Rabbit Monoclonal Antibody) validated in WB, ICC, IP to detect C/EBP β (C-term) also known as CCAAT/enhancer binding protein beta.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot detected C/EBP β in HeLa cells. Immunoprecipitation Analysis: A 1:20 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in C-terminus of rat C/EBP β.
表位	C-terminus
克隆	E299
宿主	Rabbit
特异性	This antibody can recognize three C/EBP β isoforms (i.e. LAP*, LAP, and LIP).
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_077039.3
Entrez基因汇总	transcription factor that binds to CCAATT motif on DNA and may facilitate IL-6 induced transcriptional activation
基因符号	TCF5 C/EBP-beta CRP2 IL6DBP LAP MGC32080 NF-IL6 NFIL6
纯化方法	Unpurified
UniProt编号	P17676
UniProt汇总	FUNCTION: Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation By similarity. SUBUNIT STRUCTURE: Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha, delta and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B By similarity. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed at low levels in the lung, kidney and spleen. PTM: Sumoylated by polymeric chains of SUMO2 or SUMO3. SEQUENCE SIMILARITIES: Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.

产品使用声明
质量保证	Evaluated by Western Blot on PC12 cell lysates. Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect C/EBP β in PC12 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.