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品种反应性 主要应用 宿主 格式 抗体类型 H WB, ICC, IHC M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB4146 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-BCRP Antibody, clone BXP-21 Alternate Names - ATP-binding Cassette, Sub Family G, Member 2 (ABCG2)
- Breast Cancer Resistance Protein
- Placenta-specific ATP Binding Cassette Transporter
- ATP-binding Cassette
- Sub Family G
背景信息 The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates 产品信息 格式 Culture Supernatant 控制 - MCF7 cells
演示 Mouse monoclonal IgG2a in buffer containing 0.7% BSA and 0.1% sodium azide 应用 应用 This Anti-BCRP Antibody, clone BXP-21 is validated for use in IC, IH, IH(P), WB for the detection of BCRP. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.
Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Fusion protein consisting of the E. coli maltose-binding protein and a 126-aa portion of BCRP (aa 271-396). 克隆 BXP-21 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products. 同种型 IgG2a 品种反应性 Human Species Reactivity Note Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004827.2
Entrez基因汇总 The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. 基因符号 - CDw338
- ABCG2
- BCRP
- BMDP
- BCRP1
- MXR
- ABC15
- ABCP
- EST157481
- MGC102821
- MXR1
- MRX
纯化方法 Unpurified UniProt编号 - Q9UNQ0
UniProt汇总 FUNCTION: SwissProt: Q9UNQ0 # Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123.
SIZE: 655 amino acids; 72314 Da
SUBUNIT: Monomer or homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in placenta. Low expression in small intestine, liver and colon.
PTM: Glycosylation-deficient ABCG2 is normally expressed and functional.
SIMILARITY: SwissProt: Q9UNQ0 ## Belongs to the ABC transporter family. ABCG (White) subfamily. & Contains 1 ABC transporter domain.产品使用声明 质量保证 Evaluated by Western Blot on HepG2 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For long term storage, store at -20ºC. Stable for 6 months from date of receipt at 2-8ºC. 包装信息 数量 100 µg
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