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品种反应性 主要应用 宿主 格式 抗体类型 R, M ICC, IHC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 MAB397A4 描述 Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate | MAB397A4 Alternate Names - Glutamate receptor ionotropic, AMPA 2
- Glutamate receptor 2
- GluR-2
- AMPA-selective glutamate receptor 2
- GluR-B
- GluR-K2
背景信息 Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses. 产品信息 格式 AlexaFluor®488 控制 - Adult mouse brain tissue
演示 Purified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 主要应用 - Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected GluR2 in rat E18 primary cortex cells. 生物信息 免疫原品种 Recombinant fusion protein TrpE-GluR2 表位 Extracellular domain 宿主 Mouse 特异性 Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. 同种型 IgG2a 品种反应性 RatMouse Species Reactivity Note Demonstrated to react with Mouse and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001077280
Entrez基因汇总 Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. 基因符号 - GluR-B
- Gria2
- GLUR2
- GLURB
- GluR-2
纯化方法 Protein A purified UniProt编号 - P19491
UniProt汇总 FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
SIZE: 883 amino acids; 98,688 Da
SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4 (By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.
TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.
SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.产品使用声明 质量保证 Evaluated by Immunohistochemistry in adult mouse brain tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected GluR2 in adult mouse brain tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µL
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