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merck millipore,默克密理博,MAB3757,Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9
产品名称：Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9
产品型号：MAB3757
Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9 is an antibody against VEGF Receptor-3 for use in FC, IF, IH(P), IP & WB.
merck millipore,默克密理博,MAB3757,Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB3757 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9 Alternate Names - Receptor tyrosine Kinase VEGFR-3
- VEGFR3
- VEGFR-3
背景信息 Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. {Bando, 2004}. Jussila et al reported that VEGFR3 antibodies specifically stain endothelial cells of lymphatic vessels and vessels around tumonrs such as lymphoma and in situ breast carcinoma. {Jussila, 1998}.Interestingly, the spindle cells of several cutaneous nodular AIDS-associated Kaposi's sarcomas and the endothelium around the nodules were also VEGFR-3 positive. The first specific molecular marker for the lymphatic endothelium should provide a useful tool for the analysis of lymphatic vessels in malignant tumors and their metastases and the cellular origin and differentiation of Kaposi's sarcomas. {Jussila, 1998}. 产品信息 格式 Ascites 控制 - VEGFR-3 is not expressed in HEVs of the lymph nodes. {Jussila, 1998]. In lymphomas staining is seen mostly in collapsed vessels in the cortex of lymph nodes infiltrated by the lymphoma cells. The antibody specifically detects lymphatic endothelium in human lymph node and other organs such as tonsil. See Jussila et al, 1998 for complete tissue characterization.
- Human lymphatic tissues
演示 UnPurified ascites fluid containing 0.1% sodium azide as a preservative. 应用 应用 Anti-VEGF Receptor-3 Antibody, extracellular domain, clone 9D9F9 is an antibody against VEGF Receptor-3 for use in FC, IF, IH(P), IP & WB. 主要应用 - Western Blotting
- Flow Cytometry
应用说明 Immunohistochemistry: frozen cryosections, 5μm cryosections were air-dried and fixed in cold acetone for 10 minutes. Blocked with 5% normal horse serum and then incubated with 9D9 for 2 hours at room temperature in a humid chamber. Typical dilutions will range from 1:100-1:1000 depending upon lot titer. Detection is with enhanced methods (ABC or poly HRP) and enzymatic substrates for best results.
Immunohistochemistry in paraffin: 5μm thick lightly fixed formalin tissues were processed through decreasing ethanol concentrations, washed with water, then heated in a microwave in 10mM sodium citrate pH 6.0 at 780W for 5 minutes, followed by 450W for 10 minutes. The sections were then incubated in methanol containing 30% hydrogen peroxide for 30 minutes and processed further like the cryosections. {Jussila, 1998}.
Western Blot: antibody recognizes VEGFR-3 on reduced samples of HUVEC and HDMEC cells {primay human umbilical vein endothelial cells & primary human dermal microvascular endothelial cells}. The bands seen at molecular weight 195kDa and 125kDa represent the uncleaved and proteolytically cleaved forms of VEGFR3. The band at 175kDa is considered to be intracellular, unglycosylated precursor {Bando, 2004}.
Immunoprecipitation: 1-5μL per 300-500μL of cell lysate. Use protein A or rabbit anti-mouse IgG capture for best results.
ELISA: 9D9F9 has been used as the detection antibody for a VEGFR-3 ELISA {Bando, 2004}.
Flow Cytometry: reactive on transfected NIH-3T3 cells expressing hVEGFR3, Other cell lines untested. {Jussila, 1998}.
Optimal dilutions will need to be determined by the enduser.生物信息 免疫原品种 Recombinantly produced, purified, hVEGFR-3 extracellular domain protein from baculovirus culture {Jussila, 1998}. 表位 extracellular domain 克隆 9D9F9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for human VEGFR-3. Western blot and ELISA demonstrate that the antibody is highly specific for VEGFR-3 with no cross-reactivity with human VEGFR-1 or VEGFR-2 protein {Bando, 2004}. Cross reactivity with other species VEGFR-3 proteins untested. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003376.4
- NM_001025366.1
- NM_001025367.1
- NM_001025368.1
- NM_001025369.1
- NM_001025370.1
- NM_001033756.1
Entrez基因汇总 This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. 基因符号 - VEGF-A
- VEGFA
- MGC70609
- VPF
- VEGF
纯化方法 Unpurified UniProt编号 - P15692
UniProt汇总 FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SIZE: 232 amino acids; 27042 Da
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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