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品种反应性 主要应用 宿主 格式 抗体类型 Pm, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3610 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bub1 Antibody, clone 14H5 背景信息 The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells. 产品信息 格式 Purified 演示 Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide. 应用 应用 Detect Bub1 with Anti-Bub1 Antibody, clone 14H5 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein.
Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.
Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant human Bub1. 克隆 14H5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes human Bub1. 同种型 IgG1 品种反应性 PrimateHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004336.2
Entrez基因汇总 This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. 基因符号 - EC 2.7.11.1
- BUB1
- hBUB1
- BUB1A
- BUB1L
UniProt编号 - O43683
UniProt汇总 FUNCTION: SwissProt: O43683 # Involved in cell cycle checkpoint enforcement. Can interact and phosphorylate BUB3.
SIZE: 1085 amino acids; 122375 Da
SUBCELLULAR LOCATION: Nucleus (By similarity). Kinetochore (By similarity). Note=Nuclear in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage (By similarity).
TISSUE SPECIFICITY: High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
DOMAIN: SwissProt: O43683 CD1 domain directs kinetochore localization and binding to BUB3.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:SwissProt: O43683 # Defects in BUB1 are associated with tumor formation.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. & Contains 1 CD1 domain. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µg
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