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merck millipore,默克密理博,MAB360,Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
产品名称：Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
产品型号：MAB360
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB.
merck millipore,默克密理博,MAB360,Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, Ch, H, M, Po, R WB, ICC, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB360 Replaces 04-1031; 04-1062 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 Alternate Names - GFAP
背景信息 Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. 产品信息 格式 Ascites 控制 - Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
演示 Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide. 应用 应用 Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user生物信息 免疫原品种 Purified GFAP from porcine spinal cord. 克隆 GA5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin. 同种型 IgG1 品种反应性 RabbitBovineChickenHumanMousePigRat Species Reactivity Note Reacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002055.2
Entrez基因汇总 This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined. 基因符号 - FLJ45472
- GFAP
纯化方法 Unpurified UniProt编号 - P14136
UniProt汇总 FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,MAB387-1ML,Anti-Myelin Basic Protein (84-89) Antibody
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