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merck millipore,默克密理博,MAB351,Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6
产品名称：Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6
产品型号：MAB351
Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6 detects level of Glutamate Decarboxylase & has been published & validated for use in IH & WB.
merck millipore,默克密理博,MAB351,Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB351 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6 Alternate Names - GAD65
背景信息 Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk et al., 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS. 产品信息 格式 Purified 控制 - human brain lysate
- Rat brain tissue
演示 Purified immunoglobulin. Lyophilized from 10 mM potassium phosphate, 70 mM sodium chloride, pH 7.4, 0.02% sodium azide. Reconstitute with 100 μL of sterile water (1 mg/mL). 应用 应用 Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6 detects level of Glutamate Decarboxylase & has been published & validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry: ( 1 μg/mL * See protocol)
Western blot
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB351
IMMUNOHISTOCHEMISTRY
1) Perfuse rats with 100 mM phosphate buffer, pH 7.4 containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP).
2) Postfix brains in 1% PLP for 1-2 hours.
3) Transfer brains to 100 mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at +4°C for 48-60 hours.
4) Using a sliding microtome, cut 30 mm sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100 mM phosphate buffer.
5) Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes.
6) On a shaker platform, incubate sections with MAB351 (diluted 1 μg/mL in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at +4°C.
7) On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS.
8) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
9) Mount sections, dehydrate, and apply coverslips.生物信息 免疫原品种 Purified rat brain glutamic acid decarboxylase. 克隆 GAD-6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain (Gottlieb, et al., 1986; Chang & Gottlieb, 1988). This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Anti-GAD has also been used to label purified GAD on Western blots (Chang & Gottlieb, 1988). 同种型 IgG2a 品种反应性 RatHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000818.1
Entrez基因汇总 This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. 基因符号 - EC 4.1.1.15
- GAD2
- MGC161607
- GAD65
- MGC161605
- GAD-65
纯化方法 Protein A purfied UniProt编号 - Q05329
UniProt汇总 FUNCTION: SwissProt: Q05329 # Catalyzes the production of GABA.
COFACTOR: Pyridoxal phosphate.
SIZE: 585 amino acids; 65411 Da
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
PTM: Phosphorylated; which does not affect kinetic parameters or subcellular location. & Palmitoylated; which is required for presynaptic clustering.
SIMILARITY: SwissProt: Q05329 ## Belongs to the group II decarboxylase family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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