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品种反应性 主要应用 宿主 格式 抗体类型 R, B, Ca, Ch, Fe, H, Mk, Po WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3400 Replaces MAB3410 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Vimentin Antibody, clone V9 背景信息 Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. 产品信息 格式 Purified 控制 - Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
演示 Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. 应用 应用 Anti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoblotting:
A dilution of 1:1000 was used in a previous lot.
Immunohistochemistry:
5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunocytochemistry:
5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified vimentin from pig eye lens 克隆 V9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin 同种型 IgG1 品种反应性 RatBovineCanineChickenFelineHumanMonkeyPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003380.2
Entrez基因汇总 Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] 基因符号 - FLJ36605
- VIM
非反应性品种 - Mouse
纯化方法 Protein A purfied UniProt编号 - P08670
UniProt汇总 FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SIZE: 466 amino acids; 53652 Da
SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. ...产品使用声明 质量保证 Evaluated by Western Blot on HeLa lysates.
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 40 µg
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下一件merck millipore产品：merck millipore,默克密理博,MABE339,Anti-p53 (wild type) Antibody, clone PAb1620
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