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品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ht, M FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3290 Replaces 04-575; 04-576 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-PARP Antibody, clone 7A10 Alternate Names - ARTD1
- Poly[ADP-ribose] synthase 1
- ADPRT 1
- NAD(+) ADP-ribosyltransferase 1
- PARP-1
- Poly [ADP-ribose] polymerase 1
背景信息 PARP is a highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+ -dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in the apoptosis response of cells. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro. 产品信息 格式 Purified 演示 Liquid in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 ug/mL BSA, 50% glycerol and 0.03 sodium azide. 应用 应用 Detect PARP using this Anti-PARP Antibody, clone 7A10 validated for use in WB, FC. 主要应用 - Flow Cytometry
- Western Blotting
应用说明 Western Blot (1:500-1:2000)
Flow Cytometry (1:200-1:400)
Optimal working dilutions must be determined by end user.
For Western blotting, sonication and urea are necessary to break up PARP/DNA interactions. To do this, resuspend cells in 62.5 mM Tris-HCl, pH 6.8, 6 M urea, 10% glycerol, 2% SDS, 0.00125% bromophenol blue and 5% beta-mercaptoethanol, sonicate for 15 seconds on full power and incubate at 65°C for 15 minutes, and then follow standard Western blotting procedures. Untreated cells should show only full-length PARP, while treated cells will show both cleaved and full-length PARP.生物信息 免疫原品种 A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. 克隆 7A10 宿主 Mouse 特异性 This antibody only recognizes full-length form (p116) of PARP-1.
Epitope: Region before the cleavage site of PARP-1.同种型 IgG1 品种反应性 RatHumanHamsterMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001618.3
Entrez基因汇总 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. 基因符号 - EC 2.4.2.30
- PARP1
- poly(ADP-ribosyl)transferase
- PARP-1
- PARP
- NAD
- pADPRT-1
- ADPRT1
- Poly
- ADPRT
- PPOL
UniProt编号 - P09874
UniProt汇总 FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
SIZE: 1014 amino acids; 113084 Da
SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers.
MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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