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品种反应性 主要应用 宿主 格式 抗体类型 H, R, M ICC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABS44 描述 Anti-MAPK 1/2 Antibody Alternate Names - protein tyrosine kinase ERK2
- Extracellular signal-regulated kinase 2
- MAP kinase 2
- MAPK 2
- Mitogen-activated protein kinase 2
- extracellular signal-regulated kinase-2
- mitogen-activated protein kinase 1
背景信息 The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called MAPK1 and MAPK2, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or MAPK1/2. The MAPK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of MAPK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. 产品信息 格式 Purified 控制 - A431 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect MAPK 1/2 using this Anti-MAPK 1/2 Antibody validated for use in WB, IP & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 0.5 µg/mL from a previous lot detected MAPK1/2 in BALB3T3/A31 and L6 cell lysates.
Western Blot (SNAP ID) Analysis: 1-2 µg/mL from a previous lot detected MAPK1/2 in BALB3T3/A31, L6, and A431 cell lysates.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MAPK1/2 in NIH/3T3, A431, HeLa, and C2C12 cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to rat MAPK 1/2 at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes MAPK1/2 at the C-terminus. 品种反应性 HumanRatMouse Species Reactivity Note Reacts with human, mouse, and rat. Expected to react with Bovine and Canine based on 94% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_059043
Entrez基因汇总 Kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD] 基因符号 - ERT1
- MAPK1
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41mapk
- PRKM2
- MAPK2
- p42-MAPK
- p41
- ERK-2
纯化方法 Protein A purfied UniProt编号 - P21708
UniProt汇总 FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4) By similarity.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium By similarity.
ENZYME REGULATION: Activated and tyrosine phosphorylated in response to insulin and NGF.
SUBUNIT STRUCTURE: Interacts with NISCH, MORG1 and HSF4 By similarity.
SUBCELLULAR LOCATION: Isoform 2: Nucleus.
TISSUE SPECIFICITY: Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.
DEVELOPMENTAL STAGE: Increased expression during development.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-203 and Tyr-205, which activates the enzyme.
SEQUENCE SIMLARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MAPK 1/2 on 10 µg of A431 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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