merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB328,Anti-Oligodendrocytes Antibody, clone CE-1

  • merck millipore,默克密理博,MAB328,Anti-Oligodendrocytes Antibody, clone CE-1

    产品名称：Anti-Oligodendrocytes Antibody, clone CE-1
    产品型号：MAB328
    This Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes.

    merck millipore,默克密理博,MAB328,Anti-Oligodendrocytes Antibody, clone CE-1

  • 产品介绍
  • merck millipore,默克密理博,MAB328,Anti-Oligodendrocytes Antibody, clone CE-1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Fe, Ch, H, M, Mk, RIHC, ICCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB328
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Oligodendrocytes Antibody, clone CE-1
    Alternate Names
    • MOSP
    产品信息
    格式 Ascites
    应用
    应用 This Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes.
    主要应用
    • Immunohistochemistry
    • Immunocytochemistry
    不建议的应用
    • Western Blotting
    应用说明 Immunocytochemistry

    Immunohistochemistry at 1:1,000-1:5000

    Not suggested for use in Western blot.

    Optimal working dilutions must be determined by end user.

    IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328

    This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

    1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

    2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

    3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

    4) 3 x 10 minute washes in TBS.

    5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

    6) 3 x 10 minute washes in TBS.

    7) ABC Elite (1:200 Vector Labs) in TBS.

    8) 2 x 10 minute washes in TBS.

    9) 1 x 10 minute wash in phosphate buffer (no saline).

    10) DAB reaction with 0.06% NiCl added for intensification.

    11) 2 x 10 minute washes in PBS.

    12) 1 x 10 minute wash in phosphate buffer (no saline).
    生物信息
    免疫原品种 Rat glial membranes and whole brain white matter.
    克隆 CE-1
    宿主 Mouse
    特异性 In neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994).
    同种型 IgM
    品种反应性 FelineChickenHumanMouseMonkeyRat
    抗体类型 Monoclonal Antibody
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB328,Anti-Oligodendrocytes Antibody, clone CE-1

上一件merck millipore产品：merck millipore,默克密理博,566415,PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb
下一件merck millipore产品：merck millipore,默克密理博,71745,D-Tube™ Dialyzer Mega, 20 ml, MWCO 3.5 kDa


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