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品种反应性 主要应用 宿主 格式 抗体类型 Fe, Ca, Ch, H, Ht, Mk, Po ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3242 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Smoothelin Antibody, clone R4A 背景信息 Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions. 产品信息 格式 Purified 演示 Liquid in buffer (PBS) with 0.1% sodium azide. 应用 应用 Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot
Immunocytochemistry
Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Cytoskeletal extract of chicken gizzard. 克隆 R4A 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot. 同种型 IgG1 品种反应性 FelineCanineChickenHumanHamsterMonkeyPig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_134270.1
- NM_006932.3
- NM_134269.1
Entrez基因汇总 This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants. 基因符号 - FLJ35365
- SMTN
- smoothelin
- Smoothelin.
- FLJ38597
- SMSMO
UniProt编号 - P53814
UniProt汇总 FUNCTION: SwissProt: P53814 # Structural protein (cytoskeletal).
SIZE: 917 amino acids; 99516 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Exhibits a filamentous organization.
TISSUE SPECIFICITY: Smooth muscle; contractile or vascular (for the long form).
SIMILARITY: SwissProt: P53814 ## Contains 1 CH (calponin-homology) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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