merck millipore,默克密理博,04-1121,Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal

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merck millipore,默克密理博,04-1121,Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal
产品名称：Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal
产品型号：04-1121
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect ROCK-1 using this Anti-ROCK-1 Antibody, clone More>>

merck millipore,默克密理博,04-1121,Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1121,Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1121
描述	Anti-ROCK-1 Antibody, clone EPR638Y, rabbit monoclonal
Alternate Names	p160 ROCK-1 Renal carcinoma antigen NY-REN-35 Rho-associated, coiled-coil containing protein kinase 1 Rho-associated, coiled-coil-containing protein kinase 1
背景信息	Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

产品信息
格式	Unpurified
控制	HeLa cell lysate.
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl containing 40% glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect ROCK-1 using this Anti-ROCK-1 Antibody, clone EPR638Y, Rabbit validated for use in IH(P), WB, IC & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:50-100 dilution from a previous lot detected ROCK-1 in human lymphoma tissue. Immunocytochemistry: 1:50-100 dilution from a previous lot was used in IC. Immunoprecipitation: 1:60 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the C-terminus of human ROCK-1.
表位	C-terminus
克隆	EPR638Y
宿主	Rabbit
特异性	This antibody recognizes ROCK-1.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q13464
基因符号	p160ROCK EC 2.7.11.1 MGC131603 MGC43611 OTTHUMP00000162514 P160ROCK PRO0435 p160-ROCK
纯化方法	Unpurified
UniProt编号	Q13464
UniProt汇总	FUNCTION: Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis By similarity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by RHOA binding. SUBUNIT STRUCTURE: Binds RHOA (activated by GTP). Interacts with ADD1, GEM, RHOB, RHOC, MYLC2B and VIM By similarity. Binds RHOE, PPP1R12A, LIMK1 and LIMK2. Interacts with TSG101. SUBCELLULAR LOCATIION: Cytoplasm. Centrosome › centriole By similarity. Golgi apparatus membrane; Peripheral membrane protein. Note: Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes. TISSUE SPECIFICITY: Detected in blood platelets. DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. MISCELLANEOUS: Inhibited by Y-27632. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect ROCK-1 in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.