merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3100,Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

  • merck millipore,默克密理博,MAB3100,Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

    产品名称：Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2
    产品型号：MAB3100
    Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.

    merck millipore,默克密理博,MAB3100,Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

  • 产品介绍
  • merck millipore,默克密理博,MAB3100,Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Rb, H, RWB, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB3100
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2
    Alternate Names
    • GJC1 protein
    • Cx45
    • Gap Junction gamma-1 protein
    • Gap Junction alpha-7 protein
    • Cx-45
    • GJA7 protein
    背景信息 Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.
    产品信息
    格式 Purified
    控制
    • Cardiac tissue, particularly sinoatrial tissues.
    演示 Liquid at 1 mg/mL in 0.02M PB with 0.25M NaCl,pH=7.6 containing 0.1% sodium azide as a preservative.
    应用
    应用 Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunblotting (~46 kDa): 0.5 -1 µg/mL with ECL detection. Samples should be prepared with proteinase inhibitors and kept cold for best results. Alkali extraction methods can be used to enrich for connexin protein fractions. Briefly, 1 ml of NaHCO3 containing protease and phosphatase inhibitors and 22 µl of 1 M NaOH were added to frozen cell pellets. This cell suspension was sonicated for 30 s and incubated on ice for 50 min. Then, the homogenate was centrifuged for 30 min at 30,000 X g at 4°C. The supernatant was discarded, and the pellet was resuspended in sample buffer. {Elenes, S et al 2001}. 8% SDS_PAGE gels and PVDF membranes are suggested for greatest clarity.

    Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.

    Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.


    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.
    表位 near C-terminus, cytoplasmic
    克隆 8A11.2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Clone 8A11.2 is highly specific for Connexin 45 protein with no cross reactivity as analyzed by western blotting to any other connexin family member.
    同种型 IgG1
    品种反应性 RabbitHumanRat
    Species Reactivity Note Human, rat (Sorensen, et al 2008), mouse, chicken (Elenes, et al (2001), rabbit (Chaytor, et al 2005), and dog (Wang, et al (2001), others species not tested. Also confirmed to work on rat C6 cells.
    抗体类型 Monoclonal Antibody
    基因符号
    • Connexin-45
    • ENSG00000182963
    • Cx45
    UniProt编号
    • P36383
    UniProt汇总 FUNCTION: SwissProt: P36383 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
    SIZE: 396 amino acids; 45483 Da
    SUBUNIT: A connexon is composed of a hexamer of connexins.
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
    SIMILARITY: SwissProt: P36383 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2° to 8°C in undiluted aliquots for up to 12 months fromd date of receipt. Prevent multiple warming and coolings. Aliquot in tightly closed tubes to prevent loss.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB3100,Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

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