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merck millipore,默克密理博,MAB3083,Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
产品名称：Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
产品型号：MAB3083
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
merck millipore,默克密理博,MAB3083,Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
- 产品介绍
- merck millipore,默克密理博,MAB3083,Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, B IHC, ELISA, WB, ICC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB3083 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Calpain Antibody, small subunit of µ- or m-Calpains (Calpain I or II), clone P1 背景信息 Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen. 产品信息 格式 Ascites 演示 Liquid. Contains no preservative. 应用 应用 This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
- Immunocytochemistry
应用说明 Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user. 生物信息 免疫原品种 Bovine myocardial m-calpain. 表位 small subunit of μ- or m-Calpains (Calpain I or II) 克隆 P1 宿主 Mouse 特异性 Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II). 同种型 IgG1 品种反应性 HumanBovine 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001749.2
- NM_001003962.1
Entrez基因汇总 Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene. 基因符号 - CALPAIN4
- CAPNS1
- CDPS
- 30K
- CANPS
- CAPNS
- CANP
- CSS1
- CAPN4
非反应性品种 - Rat
UniProt编号 - P04632
UniProt汇总 FUNCTION: SwissProt: P04632 # Regulatory subunit of the calcium-regulated non- lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
COFACTOR: Calcium (By similarity).
SIZE: 268 amino acids; 28316 Da
SUBUNIT: Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocates to the plasma membrane upon calcium binding (By similarity).
DOMAIN: SwissProt: P04632 The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer (By similarity).
SIMILARITY: Contains 5 EF-hand domains.
MISCELLANEOUS: EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF- hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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