- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB2221,Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae
产品名称：Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae
产品型号：MAB2221
This Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae is validated for use in FC, IP, WB, IH for the detection of MHC Class II.
merck millipore,默克密理博,MAB2221,Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae
- 产品介绍
- merck millipore,默克密理博,MAB2221,Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB2221 Replaces CBL1324 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae 背景信息 MHC class I and class II molecules transport foreign and self peptides to the cell surface and present them to T lymphocytes. Detection of these peptide-MHC complexes has thus far been limited to analysis of the response of a T cell. mAb Y-Ae reacts with 10 to 15% of class II molecules on peripheral B lymphocytes and on cells in the thymus medulla, but not thymus cortex in mice, that express both I-Ab and I-Eb molecules. Y-Ae also detects a self E alpha peptide bound to I-Ab molecules. It does not recognize I-ab alone. It does not bind to the peptide groove and block antigen presentation and thus it is a useful tool in tracking antigen presentation because it only binds when the correct peptide + MHC is present. In addition to B lymphocytes, the Y-Ae determinant is expressed at comparable levels on other antigen-presenting cells, including macrophages and dendritic cells. Finally, the antibody does not react with invariant chain-associated class II complexes, thus providing direct evidence that invariant chain-class II complexes and peptide-class II complexes are mutually exclusive. 产品信息 格式 Purified 演示 Liquid in 0.2M phosphate, 0.25M NaCl, pH 7.6, containing no preservatives. 应用 应用 This Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae is validated for use in FC, IP, WB, IH for the detection of MHC Class II. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
应用说明 Western Blot
Immunohistochemistry: 1-2 μg/mL on frozen tissue with acetone fixation
Immunoprecipitation
Flow Cytometry: 1-2 μg per 10E6 cells in 100μL volume
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 5R - LPS B cell blasts 表位 contains Ealpha peptide 52-68, no azide 克隆 Y-Ae 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Y-Ae is specific to mouse MHC Class II IAb molecule containing the 52-68 peptide of the alpha chain of I-E peptide. This antibody is capable labelling antigen presentation because it specifically binds to the binding site when peptide is present. 同种型 IgG2bκ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002111.6
Entrez基因汇总 Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. 基因符号 - HD
- HTT
- IT15
- SLC6A4
- Huntingtin
- OCD1
UniProt编号 - P42858
UniProt汇总 FUNCTION: SwissProt: P42858 # May play a role in microtubule-mediated transport or vesicle function.
SIZE: 3144 amino acids; 347860 Da
SUBUNIT: Binds SH3GLB1 (By similarity). Interacts through its N- terminus with FNBP3. Interacts with PQBP1, SETD2 and SYVN.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
PTM: Cleaved by apopain downstream of the polyglutamine stretch. The resulting amino-terminal fragment is cytotoxic and provokes apoptosis. & Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
DISEASE: SwissProt: P42858 # Defects in HD are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
SIMILARITY: SwissProt: P42858 ## Belongs to the hungtintin family. & Contains 10 HEAT repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µg
上一件merck millipore产品：merck millipore,默克密理博,SF-1096,miR-125b-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,14-936M,NEK9, active
相关产品
merck millipore,默克密理博,178375,APO-10 - CAS 2190-95-6 - Calbiochem
merck millipore,默克密理博,219368,Carcinoembryonic Antigen, Human Tumor
merck millipore,默克密理博,MABE227,Anti-FTO Antibody, clone 5-2H10
merck millipore,默克密理博,553212,InSolution™ Rapamycin in EtOH
merck millipore,默克密理博,SF-1096,miR-125b-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,14-936M,NEK9, active
merck millipore,默克密理博,S7108,ApopTag Stop/Wash Buffer
merck millipore,默克密理博,HTS083RTA,Ready-to-Assay™ CCK2 Cholecystokinin Receptor Frozen Cells
merck millipore,默克密理博,06-918,Anti-Rsk2/MAPKAP Kinase 1b Antibody