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merck millipore,默克密理博,MAB2077Z,Anti-Integrin αVβ6 Antibody, clone 10D5, azide free
产品名称：Anti-Integrin αVβ6 Antibody, clone 10D5, azide free
产品型号：MAB2077Z
Anti-Integrin αVβ6 Antibody, clone 10D5, azide free is an antibody against Integrin αVβ6 for use in FC & IP.
merck millipore,默克密理博,MAB2077Z,Anti-Integrin αVβ6 Antibody, clone 10D5, azide free
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB2077Z 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin αVβ6 Antibody, clone 10D5, azide free Alternate Names - MAB2077
背景信息 The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide.
The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation.产品信息 格式 Purified 控制 - HT-29 colon carcinoma cells
演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives. 应用 应用 Anti-Integrin αVβ6 Antibody, clone 10D5, azide free is an antibody against Integrin αVβ6 for use in FC & IP. 主要应用 - Flow Cytometry
不建议的应用 - Immunohistochemistry
- Western Blotting
应用说明 Flow cytometry: Effective for mouse and human.
Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture
Function blocking: 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL.
Not effective for western blot or immunohistochemistry.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Immunization of beta 6 knockout mice with wild type murine keratinocytes. 克隆 10D5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Monoclonal antibody MAB2077Z, raised by immunizing beta6 knockout mice with wild type murine keratinocytes, recognizes human and murine alphaVbeta6 by flow cytometry and immunoprecipitation and has blocking activity against murine and human alphaVbeta6. 100% inhibition of alphaVbeta6-mediated cell adhesion and cell migration are achieved at a concentration of <10 μg/mL. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species, however it has been evaluated only in human and mouse.The antibody is specific for alphaVbeta6 and does not recognize aV or other aV integrins. 同种型 IgG2a 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002210.2
Entrez基因汇总 ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. 基因符号 - VNRA
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
纯化方法 Protein A purfied UniProt编号 - P06756
UniProt汇总 FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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