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品种反应性 主要应用 宿主 格式 抗体类型 Rb, Gp, H WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB19101 Replaces MAB1902 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tenascin Antibody, clone DB7 Alternate Names - Tenascin-C
产品信息 格式 Purified 演示 The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide. 应用 应用 Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.生物信息 免疫原品种 Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography. 克隆 DB7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein 同种型 IgG2a 品种反应性 RabbitGuinea PigHuman 抗体类型 Monoclonal Antibody 基因符号 - MAC-1
- ITGAM
- MO1A
- CD11b
- CR3A
- MAC1A
- CD11B
- MGC117044
非反应性品种 - Rat
- Mouse
UniProt编号 - P24821
UniProt汇总 FUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
SIZE: 1152 amino acids; 127179 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C 包装信息 数量 50 µg
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