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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB1664 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-VEGF Receptor-1 Antibody Alternate Names - FLT-1
产品信息 格式 Purified 演示 Purified from Ascites. Liquid in PBS and 5% Trehalose. 应用 应用 Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 0.5-2.0 μg/mL. MW = 180 kDa.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002019.3
Entrez基因汇总 Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM] 基因符号 - EC 2.7.10.1
- FLT1
- VEGFR1
- FLT
- FRT
- Flt-1
- VEGFR-1
UniProt编号 - P17948
UniProt汇总 FUNCTION: SwissProt: P17948 # Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
SIZE: 1338 amino acids; 150769 Da
SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.
SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted.
TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw. 包装信息 数量 50 µL
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