merck millipore,默克密理博,MAB1621,Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Ma, Av	IHC, WB, ICC	M	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MAB1621
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11
背景信息	Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

产品信息
格式	Culture Supernatant

应用
应用	Anti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections Immunoblotting: 1:1000 - 1:2000 ECL detection. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.
表位	C-terminus
克隆	3H11
宿主	Mouse
特异性	Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.
同种型	IgG1
品种反应性	MammalsAvian
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_005382.2 NM_001105541.1
基因符号	NFM NEFM NF-M NEF3
UniProt编号	P07197
UniProt汇总	FUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. SIZE: 916 amino acids; 102448 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

包装信息
数量	300 µL

merck millipore,默克密理博,MAB1621,Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

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