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merck millipore,默克密理博,04-914,Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal
产品名称：Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal
产品型号：04-914
Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4.
merck millipore,默克密理博,04-914,Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-914 Replaces 05-914 描述 Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal Alternate Names - mothers against decapentaplegic, Drosophila, homolog of, 4
- Deletion target in pancreatic carcinoma 4
- MAD, mothers against decapentaplegic homolog 4
- MAD, mothers against decapentaplegic homolog 4 (Drosophila)
- Mothers against DPP homolog 4
- SMAD 4
- SMAD family member 4
- SMAD, mothers against DPP homolog 4
- SMAD, mothers against DPP homolog 4 (Drosophila)
- deleted in pancreatic carcinoma locus 4
- mothers against decapentaplegic homolog 4
背景信息 Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies. 产品信息 格式 Purified 控制 - RIPA lysates from L6 cells
演示 Cultured supernantant containing 0.05% sodium azide. 应用 应用 Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4. 主要应用 - Western Blotting
生物信息 免疫原品种 Fusion protein corresponding to amino acids 186-273 of human Smad2. 表位 a.a. 186-273 克隆 C4T 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa. 品种反应性 RatHumanMouse Species Reactivity Note Human. Predicted to cross-react with mouse and rat based on conservation of immunogen sequence. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001003652.1, NP_005893.1
Entrez基因汇总 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional
modulators that mediate multiple signaling pathways. This protein
mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is
recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors.This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively
spliced transcript variants encoding the same protein have been observed.基因符号 - hSMAD4
- DPC4
- JIP
- MADH4
- OTTHUMP00000163548
UniProt编号 - Q13485
UniProt汇总 FUNCTION:Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor.
SUBUNIT STRUCTURE: May form trimers with receptor-regulated SMAD (R-SMAD). Found in a ternary complex composed of SMAD4, STK11 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11, STK11IP and TRIM33. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with USP9X.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD.
PTM: Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade.
INVOLVEMENT IN DISEASE: Defects in SMAD4 are a cause of pancreatic carcinoma [MIM:260350].
Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers.
Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis (JIP) and hereditary hemorrhagic telangiectasia (HHT) [MIM:187300] in a single individual. JIP and HHT are autosomal dominant disorders with distinct and non-overlapping clinical features. The former, an inherited gastrointestinal malignancy predisposition, is caused by mutations in SMAD4 or BMPR1A, and the latter is a vascular malformation disorder caused by mutations in ENG or ACVRL1. All four genes encode proteins involved in the transforming-growth-factor-signaling pathway. Although there are reports of patients and families with phenotypes of both disorders combined, the genetic etiology of this association is unknown.
Defects in SMAD4 may be a cause of colorectal cancer (CRC) [MIM:114500].
SEQUENCE SIMILARITIES:Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.产品使用声明 质量保证 Evaluated by Western Blot on L6 lysates.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µl
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