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品种反应性 主要应用 宿主 格式 抗体类型 H IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB13414 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-7 Antibody, clone ID-2 Alternate Names - PUMP-1
- Matrilysin
背景信息 Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials (e.g., 4-aminophenylmercuric acetate, APMA) and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, pancreatic carcinomas and approximately 50% of gliomas. 产品信息 格式 Purified 控制 - POSITIVE CONTROL: Bladder, breast, and ovarian carcinomas.
演示 Purified from ascites fluid by Protein A chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide. 应用 应用 This Anti-MMP-7 Antibody, clone ID-2 is validated for use in IF, IH, IH(P) for the detection of MMP-7. 主要应用 - Immunohistochemistry
应用说明 Immunofluorescence
Immunohistochemistry on frozen and formalin-fixed paraffin embedded tissue sections: 1:100-1:200 for 60 minutes at room temperature*.
*No pretreatment /antigen retrieval required for staining routine formalin-fixed, paraffin embedded sections.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant human matrilysin. 克隆 ID-2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Antibody recognizes proteins of ~28 kDa and ~18 kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-7. The antibody shows no cross-reaction with the pro and active forms of other MMPs.
Cellular Localization: Cytoplasmic (Visscher et al., 1994).同种型 IgG2bκ 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002423.3
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. 基因符号 - EC 3.4.24.23
- MMP7
- MPSL1
- matrin
- MMP-7
- PUMP-1
- Matrin
- PUMP1
UniProt编号 - P09237
UniProt汇总 FUNCTION: SwissProt: P09237 # Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
COFACTOR: Binds 2 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 267 amino acids; 29677 Da
DOMAIN: SwissProt: P09237 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. 包装信息 数量 100 µg
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