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merck millipore,默克密理博,MAB1120,Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
产品名称：Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
产品型号：MAB1120
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.
merck millipore,默克密理博,MAB1120,Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
- 产品介绍
- merck millipore,默克密理博,MAB1120,Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IP, IH(P) M Purified Monoclonal Antibody 描述 产品目录编号 MAB1120 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 Alternate Names - CD221
- IGF-IR
产品信息 格式 Purified 演示 Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP. 主要应用 - Immunoprecipitation
- Immunohistochemistry (Paraffin)
应用说明 Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA. 克隆 24-31 宿主 Mouse 特异性 Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
Weakly reacts with Rabbit.同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000875.3
Entrez基因汇总 This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. 基因符号 - Insulin-like growth factor 1 receptor beta chain].
- IGF1R
- MGC142170
- MGC18216
- IGFIR
- JTK13
- MGC142172
- CD221
- EC 2.7.10.1 [Contains: Insulin-like growth factor 1 receptor alpha chain
UniProt编号 - P08069
UniProt汇总 FUNCTION: SwissProt: P08069 # This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.
SIZE: 1367 amino acids; 154793 Da
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin- like growth factor I (IGF I). & Phosphorylation of Tyr-980 is required for IRS1- and SHC1- binding.
DISEASE: SwissProt: P08069 # Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
SIMILARITY: SwissProt: P08069 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 3 fibronectin type-III domains. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 100 µg
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