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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB19036 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-ADAM 15 Antibody, CT Alternate Names - MDC15
- Metargidin
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 50% glycerol and 0.05% sodium azide 应用 应用 This Anti-ADAM 15 Antibody, C-terminus is validated for use in WB for the detection of ADAM 15. 主要应用 - Western Blotting
应用说明 Western blot: 1:500 Optimal working dilutions must be determined by end user. 生物信息 免疫原品种 A synthetic peptide derived from the C-terminal end of the cytoplasmic domain of human ADAM15 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The ADAM (A Disintegrin and Metalloprotease) family of cell surface proteases mediate a number of cellular processes, including differentiation, cell fusion, cell-cell interactions, and shedding of plasma membrane-bound growth factor precursors1. Each ADAM contains a prodomain, a catalytic metalloprotease domain, a disintegrin domain, a transmembrane domain, and a cytoplasmic domain1. The cytoplasmic domain of ADAM15 is phosphorylated and interacts with Src family tyrosine kinases2. ADAM15 contains an RGD sequence within its disintegrin domain, and thus has been given the trivial name metargidin. In HUVEC, ADAM15 localizes to adherens junctions3. Although the mature ADAM15 has a molecular weight of 100 kD, ADAM family members can undergo proteolytic processing autocatalytically or by furin proteases to yield smaller molecular weight forms. This antibody recognizes the 100 kD full-length form in HUVEC lysates, but other forms are possible in different cell and tissue types. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_207197.1
- NM_003815.3
- NM_207191.1
- NM_207194.1
- NM_207195.1
- NM_207196.1
Entrez基因汇总 The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - EC 3.4.24.-
- ADAM15
- MDC-15
- metargidin
- Metargidin
- MDC15
UniProt编号 - Q13444
UniProt汇总 FUNCTION: SwissProt: Q13444 # May be involved in cell-surface proteolysis, cell adhesion or intracellular protein maturation.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SIZE: 814 amino acids; 87717 Da
SUBUNIT: Interacts with ITAGV-ITGB3 (vitronectin receptor), PACSIN3 and SNX9. PACSIN3 and SNX9 preferentially bind the precursor but not the processed form of ADAM15, suggesting that the interaction occurs in a secretory pathway compartment prior to the medial Golgi (By similarity). Interacts specifically with Src family protein-tyrosine kinases (PTKs). Interacts with SH3PXD2A.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes.
DOMAIN: SwissProt: Q13444 The cytoplasmic domain interacts with endophilin I and sorting nexin 9 (By similarity). & Desintegrin domain binds to integrin alphaV-beta3. & The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity). & Phosphorylation increases association with PTKs.
SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C for up to 12 months. 包装信息 数量 100 µg
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