merck millipore,默克密理博,MAB1012,Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
E. coli	ICC, WB	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MAB1012
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only
Alternate Names	Alk. Phos. AP

产品信息
格式	Ascites
演示	Ascites. Contains no preservative.

应用
应用	Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only detects level of Alkaline Phosphatase & has been published & validated for use in IP, WB & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Western blot at 1:5,000. 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein. Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested. ASSAY: Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991). Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991). Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	F201C1B
表位	E. coli, bacterial only
宿主	Mouse
特异性	Alkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western blot analysis of E. coli transformants expressing fusion products.
同种型	IgG2a
品种反应性	E. coli
抗体类型	Monoclonal Antibody
Entrez基因编号	945041
Entrez基因汇总	Alkaline phosphatases are non-specific phosphomonoesterases that catalyze the hydrolysis reaction via a phosphoseryl intermediate to produce inorganic phosphate and the corresponding alcohol, optimally at high pH. Alkaline phosphatase exists as a dimer, each monomer binding 2 zinc atoms and one magnesium atom, which are essential for enzymatic activity.
UniProt编号	P00634
UniProt汇总	FUNCTION: SwissProt: P00634# alkaline phosphatase activity COFACTOR: Binds 1 magnesium ion; Binds 2 zinc ions. SIZE: 471 amino acids; 49,439 Da SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Periplasm, Periplasmic space. PTM: Disulfide bond; Phosphoprotein. SIMILARITY: SwissProt: P00634 ## Belongs to the alkaline phosphatase family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

包装信息
数量	100 µL

merck millipore,默克密理博,MAB1012,Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech