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merck millipore,默克密理博,HTS181M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW2 Neuropeptide B/W Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW2 Neuropeptide B/W Receptor
产品型号：HTS181M
Human NPBW2 / GPR8 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS181M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW2 Neuropeptide B/W Receptor
- 产品介绍
- merck millipore,默克密理博,HTS181M,ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW2 Neuropeptide B/W Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 NPBW2 / GPR8 描述 产品目录编号 HTS181M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human NPBW2 Neuropeptide B/W Receptor 概述 Full length human GPR8 cDNA encoding NPBW2 背景信息 Neuropeptide B (NPB) and neuropeptide W (NPW) are members of a recently identified neuropeptide family that are ligands for NPBW1 and NPBW2 receptors, both of which are coupled to Gi/o protein to inhibit intracellular cAMP production and share 64% sequence homology (Singh and Davenport, 2006). NPBW1 recognizes both NPB and NPW with similar nanomolar affinities (with a slight preference for NPB), whereas NPBW2 is moderately selective for NPW (Tanaka et al., 2003). NPBW2 is one of a few GPCRs that have no rat or mouse orthologue, although gene encoding NPBW2 has been discovered in other mammalian species such as rabbit (Lee et al., 1999). NPBW2 mRNA is known to be expressed in the frontal cortex, parietal cortex hippocampus, caudate nucleus, thalamus, pituitary, adrenal gland, and lymph node (Brezillon et al., 2003). Functions of NPBW2 may be involved in feeding, weight regulation, and pain response through direct or indirect actions in the central nervous system. Millipore's NPBW2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NPBW2 receptor interactions with its ligand. The membrane preparations exhibit a Kd of 2nM for [125I]-Neuropeptide W23. With 10 ug/well NPBW2 Membrane Prep and 0.35nM [125I]-Neuropeptide W23, a greater than 10-fold signal-to-background ratio was obtained. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.应用 应用 Human NPBW2 / GPR8 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_005286.2
Entrez基因汇总 The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein is similar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid and somatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarily in the frontal cortex of the brain. 基因符号 - GPR8
- NPBWR2
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 NPBW2 / GPR8 靶子系列 Neuropeptide B/W UniProt编号 - P48146
UniProt汇总 FUNCTION: SwissProt: P48146 # Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals.
SIZE: 333 amino acids; 36861 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in the adult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes.
SIMILARITY: SwissProt: P48146 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C.
Radioligand: [125I]-neuropeptide W23 (Perkin Elmer#: NET-403)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled neuropeptide W23 at 0.35nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with 10 or 20 µg/well of NPBW2 Receptor membrane prep. 20 µg/well 10 µg/well Signal:Background 26.3 30.2 Specific Binding (cpm) 20148 19282
SPECIFICATIONS: 1 unit = 10.0 µg membrane preparation
Bmax: 11.34 pmol/mg
Kd: 2 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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