merck millipore,默克密理愽
  • merck millipore,默克密理博,HTS138M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

  • merck millipore,默克密理博,HTS138M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

    产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor
    产品型号：HTS138M
    Human CXCR7 / RDC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

    merck millipore,默克密理博,HTS138M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

  • 产品介绍
  • merck millipore,默克密理博,HTS138M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

    重要规格表

    应用说明 品种 宿主细胞 蛋白靶
    Radioligand binding assay and GTPγS bindingHumanChem-1CXCR7 / RDC1
    描述
    产品目录编号 HTS138M
    商名
    • Chemicon
    • ChemiScreen
    描述 ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor
    概述 : Full-length human CXCR7 cDNA encoding CXCR7
    Alternate Names
    • RDC1
    背景信息 The orphan receptor RDC1 is phylogenetically related to chemokine receptors, and its gene (CMKOR1, chemokine orphan receptor 1) occupies a chromosomal location near the genes for other CXC receptors. Several immune system cells, including T lymphocytes, mature dendritic cells and memory B cells, express RDC1 (Infantino et al., 2006). A subset of HIV and SIV strains use RDC1 as a coreceptor (Shimizu et al., 2000). The chemokine SDF-1/CXCL12, which binds to CXCR4, has been shown also to bind and activate RDC1 to promote chemotaxis of T lymphocytes; thus, CXCR7 has been proposed to be the new name for RDC1 (Balabanian et al., 2005). I-TAC, one of the chemokines thought to bind to CXCR3 only, has been reported to distinguish CXCR7 from CXCR4 in radioligand binding experiments (Burns et al. 2006). Chemicon's CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-to-background ratio.
    产品信息
    演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
    Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.
    应用
    应用 Human CXCR7 / RDC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
    应用说明 Radioligand binding assay and GTPγS binding
    生物信息
    品种 Human
    Entrez基因编号
    • NM_020311.2
    Entrez基因汇总 This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.
    基因符号
    • RDC1
    • CXCR7
    • CXCR-7
    • GPR159
    • CMKOR1
    • RDC-1
    • CXC-R7
    GPCR类别 A
    宿主细胞 Chem-1
    蛋白靶 CXCR7 / RDC1
    靶子系列 Chemokine
    UniProt编号
    • P25106
    UniProt汇总 FUNCTION: SwissProt: P25106 # Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
    SIZE: 362 amino acids; 41493 Da
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in monocytes, basophils, and B- cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
    SIMILARITY: SwissProt: P25106 ## Belongs to the G-protein coupled receptor 1 family.
    孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
    Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
    Radioligand: [125I] SDF-1α (Perkin Elmer#: NEX-346)
    Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.

    One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled SDF-1α at 0.25 nM
    产品使用声明
    质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with CXCR7 membrane prep and unlabeled SDF-1α.
    10 µg/well
    Signal:Background 4.92
    Specific Binding (cpm) 15919

    1 unit = 10 µg
    Bmax for [125I]- SDF-1α binding: 0.51 pmol/mg protein
    Kd for [125I]- SDF-1α binding: ~0.32 nM
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
    包装信息
    数量 200 units

    merck millipore,默克密理博,HTS138M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor

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