merck millipore,默克密理博,HTS123M,ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation

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merck millipore,默克密理博,HTS123M,ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation
产品名称：ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation
产品型号：HTS123M
Human BB1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

merck millipore,默克密理博,HTS123M,ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS123M,ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPγS binding.	Human	Chem-1	BB1

描述
产品目录编号	HTS123M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation
概述	Full-length human NMBR cDNA encoding BB1
背景信息	Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB). A family of 3 GPCRs, including NMB-R (BB₁), GRP-R (BB₂), and BRS-3 (BB₃), mediate the biological effects of the peptides. The receptors differ in their affinities for the peptides; BB2 binds to GRP with 50-300-fold greater affinity than to NMB, whereas BB1 binds to NMB with 10-800-fold greater affinity than to GRP (Tokita et al., 2004). Binding of ligand to BB1 activates Gq to increase intracellular calcium concentrations. The CNS is a major site of NMB and BB1 expression, and BB₁ appears to be involved in thermoregulation (Ohki-Hamazaki et al., 2005). Chemicon's BB₁ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of BB1 interactions with neuromedin B. The membrane preparations exhibit a Kd of 0.35-0.43 nM for [¹²⁵I]-Bombesin. With 5 µg/well BB₁ Membrane Prep and 0.3 nM [¹²⁵I]-bombesin, a greater than 40-fold signal-to-background ratio was obtained.

产品信息
演示	One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 40-fold signal:background with ¹²⁵I-labeled bombesin at 0.3 nM. Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

产品信息

演示

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 40-fold signal:background with ¹²⁵I-labeled bombesin at 0.3 nM.

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human BB1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay, and GTPγS binding.

生物信息
品种	Human
Entrez基因编号	NM_002511.2
Entrez基因汇总	Neuromedin B receptor binds neuromedin B, a potent mitogen and growth factor for normal and neoplastic lung and for gastrointestinal epithelial tissue.
基因符号	NMB-R NMBR
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	BB1
靶子系列	Bombesin
UniProt编号	P28336
UniProt汇总	FUNCTION: SwissProt: P28336 # Receptor for neuromedin-B. SIZE: 390 amino acids; 43453 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P28336 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl₂, 1 mM CaCl₂, 0.2% BSA, filtered and stored at 4°C Radioligand: [¹²⁵I] bombesin (Perkin Elmer # NEX258) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of BB₁ membrane prep:

	10 µg/well	5 µg/well
Signal:Background	82.5	74.8
Specific Binding (cpm)	27377	22443

SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 3.1 pmol/mg
Kd: 0.39 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

包装信息
数量	200 units
包装	1 mL per vial

merck millipore,默克密理博,HTS123M,ChemiScreen™ Recombinant Human BB1 Bombesin/Neuromedin B Receptor Membrane Preparation

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