merck millipore,默克密理博,HTS110RTA,Ready-to-Assay™ 5-HT4B Serotonin Receptor Frozen Cells

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merck millipore,默克密理博,HTS110RTA,Ready-to-Assay™ 5-HT4B Serotonin Receptor Frozen Cells
产品名称：Ready-to-Assay™ 5-HT4B Serotonin Receptor Frozen Cells
产品型号：HTS110RTA
Human 5-HT4 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS110RTA,Ready-to-Assay™ 5-HT4B Serotonin Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS110RTA,Ready-to-Assay™ 5-HT4B Serotonin Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	5-HT4

描述
产品目录编号	HTS110RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ 5-HT_4B Serotonin Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. 5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT4 comprises at least 8 isoforms varying at the C-terminus, which are generated by alternative splicing. The expression and distribution of these splice variants differs among organs and tissues with many of them present in several tissues such as atrium, brain, and GI tract (Bockaert et al. 2004). To date, all isoforms have been shown to activate adenylyl cyclase in vitro, and no difference in signal transduction between C-terminal 5-HT4 receptor variants has been demonstrated. 5-HT4 receptors are of potential interest for the treatment of patients with GI motility disorders and Alzheimer's disease (De Maeyer et al. 2008; Lezoualc’h 2007). Millipore’s cloned human 5HT4B-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant 5HT4B expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at 5HT4B Receptor.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human 5-HT4 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_000870
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	5-HT4
靶子系列	Serotonin
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.