merck millipore,默克密理博,AB16901,Anti-Green Fluorescent Protein Antibody

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merck millipore,默克密理博,AB16901,Anti-Green Fluorescent Protein Antibody
产品名称：Anti-Green Fluorescent Protein Antibody
产品型号：AB16901
This Anti-Green Fluorescent Protein Antibody is validated for use in IC, IP, WB for the detection of Green Fluorescent Protein.

merck millipore,默克密理博,AB16901,Anti-Green Fluorescent Protein Antibody

产品介绍

merck millipore,默克密理博,AB16901,Anti-Green Fluorescent Protein Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt	WB, ICC	Ch	Purified	Polyclonal Antibody

描述
产品目录编号	AB16901
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Green Fluorescent Protein Antibody
Alternate Names	eGFP GFP
背景信息	The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

产品信息
格式	Purified
控制	GFP fusion protein expressed in cells
演示	Purified chicken immunoglobulin in TBS, pH 7.5 with 0.02% sodium azide.

应用
应用	This Anti-Green Fluorescent Protein Antibody is validated for use in IC, IP, WB for the detection of Green Fluorescent Protein.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoblotting: AB16901 detects a band of the proper molecular weight (30kDa) in lysates from E.coli expressing GFP but not in E.coli that do not express or in lysates prepared from E.coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein. Did not detect any signal when 50μg of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE. Titers were 10-20,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB. Immunocytochemistry: GFP can be detected in cells that are transfected with a plasmid directing the expression of either GFP or a GFP-Fusion protein. Cells that do not express GFP exhibit no detectable staining. AB binding was determined using HRP conjugated-anti chicken secondary Abs and visualized using DAB. The anti-GFP Abs were used at 1-2,500-5,000 fold dilution. Cells were fixed using 4% formaldehyde in PBS (pH7.4). For immunocytochemistry, the cultures were fixed in 4% formaldehyde in PBS pH7.4, for 20 minutes at room temperature. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the aminal in which the secondary antibody was raised) in PBS for 20 minutes at room temperature. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight. Antibody binding is detected using an avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine / 0.02% hydrogen peroxide solution on ice for 8-10 minutes. AB16901 has been used to detect GFP in primary neurons and zebrafish fibroblasts. Immunoprecipitation: 5μg antibody per no more than 500μl of cell lysate. It is important to use a rabbit anti-chicken secondary for capture or anti-chicken beads as Chicken IgG will not react with protein A or protein G. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	A recombinant green fluorescent protein (GFP) containing a 6-his tag, was highly purified by affinity chromatography on Nickel column followed by chromatography on a P-100 gel filtration column. The resulting product was present as only a singl
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Chicken
特异性	AB16901 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources.
品种反应性	Vertebrates
抗体类型	Polyclonal Antibody
纯化方法	Ammonium sulfate precipitation
UniProt编号	P42212

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8°C. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

储存和货运信息

存储条件

The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8°C.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.