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merck millipore,默克密理博,HTS055M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor
产品型号：HTS055M
Human CXCR5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS055M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor
- 产品介绍
- merck millipore,默克密理博,HTS055M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-5 CXCR5 描述 产品目录编号 HTS055M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR5 Chemokine Receptor 概述 Human CXCR5 背景信息 CXCR5 (also known as BLR1) is a GPCR that binds selectively to the chemokine CXCL13 (also known as BLC and BCA-1) to mediate immune system development and function (Förster et al., 1996; Gunn et al., 1998). CXCR5 cooperates with CCR7 to determine functional organization of lymphoid organs such as lymph nodes and Peyer’s patches (Ohl et al., 2003). In addition, CXCR5 mediates migration of B cells into the follicles of the splenic white pulp, thus permitting antigen deposition on follicular dendritic cells (Förster et al., 1996; Cinamon et al., 2008). Patients with rheumatoid arthritis (RA) displayed upregulated expression of CXCR5 to a greater extent than other chemokine receptors, and CXCR5-null mice subjected to antigen-induced arthritis display reduced joint destruction (Schmutz et al, 2004; Wengner et al, 2007). Therefore, CXCR5 represents a potential target for treatment of rheumatoid arthritis. Millipore's CXCR5 Membrane Preparation for GTPγS Assay is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s CXCR5 Calcium Optimized Cell Line (HTS055C). This CXCR5 Membrane Preparation has been optimized for low basal activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist and antagonist activities. The membrane preparations exhibits EC50 of 1.8 nM for CXCL13 in a GTPγS binding assay. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human CXCR5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_001716
GPCR类别 A 宿主细胞 Chem-5 蛋白靶 CXCR5 靶子系列 Chemokine 孵化条件 Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled CXCL13 was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
One package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL13-stimulated [35S]-GTPγS binding.产品使用声明 质量保证 1 unit = 5 μg
EC50 in GTPγS binding assay by CXCL13: ~ 1.8 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
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