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merck millipore,默克密理博,FCCH100109,FlowCellect MitoStress Kit
产品名称：FlowCellect MitoStress Kit
产品型号：FCCH100109
This FlowCellect MitoStress Kit for Flow Cytometry includes MitoSOX Red, a live-cell permeant fluorogenic dye & Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of a More>>
merck millipore,默克密理博,FCCH100109,FlowCellect MitoStress Kit
- 产品介绍
描述 产品目录编号 FCCH100109 商名 - FlowCellect
描述 FlowCellect MitoStress Kit 概述 Millipore’s FlowCellect™ MitoStress Kit includes MitoSOX Red, a live-cell permeant fluorogenic dye which targets the mitochondria and reacts with superoxide radicals and fluoresces yellow/red, and Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells. The simultaneous use of these reagents allows researchers to obtain information on oxidative stress and apoptosis in one simple assay.
MitoSOX Red is excitable by a 488nm laser and fluoresces maximally at 580 nm (Yellow fluorescence on the guava easyCyte 8HT). Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF647 which is excited by a red laser and emits at 665 nm (Red2 fluorescence on the guava easyCyte 8HT). Control cells depict no Red2 fluorescence while apoptotic cells exhibit positive Red2 fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with little or no superoxide 2) Stressed cells with oxidized MitoSOX Red (indicator of accumulated superoxide) but no Annexin V staining 3) Stressed and Early Apoptotic cells with oxidized MitoSOX Red and Annexin V binding and 4) Apoptotic Cells with Annexin V binding only., The MitoStress kit rapidly provides information on correlation of mitochondrial superoxide stress with apoptosis using minimal cellular sample. The entire assay can be performed in less than 30 minutes, once cellular samples are ready.Alternate Names - FlowCellect
产品信息 部件 - User Manual
- MitoSOX Red
- Annexin V CF647
- 10X Assay Buffer
检测方法 Fluorescent 应用 应用 This FlowCellect MitoStress Kit for Flow Cytometry includes MitoSOX Red, a live-cell permeant fluorogenic dye & Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells. 主要应用 - Flow Cytometry
生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 100 Tests
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