merck millipore,默克密理博,HTS043M,ChemiScreen™ VPAC1 Membrane Preparation

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merck millipore,默克密理博,HTS043M,ChemiScreen™ VPAC1 Membrane Preparation
产品名称：ChemiScreen™ VPAC1 Membrane Preparation
产品型号：HTS043M
Human VPAC1 / VIP1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

merck millipore,默克密理博,HTS043M,ChemiScreen™ VPAC1 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS043M,ChemiScreen™ VPAC1 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPgammaS binding.	Human	Chem-1	VPAC1 / VIP1

描述
产品目录编号	HTS043M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiScreen™ VPAC₁ Membrane Preparation
概述	Human VIPR1 cDNA, encoding VPAC1
背景信息	Vasoactive intestinal peptide (VIP), a 28 amino acid peptide originally isolated by its vasodilation activity, binds to two class B GPCRs, VPAC1 and VPAC2, to exert its functions in the CNS, vasculature, immune system and adrenal medulla (Harmar et al., 1998). In the immune system, VIP is synthesized by mast cells and lymphocytes, and appears to inhibit inflammation and to shift the immune response toward a Th2 pathway (Delgado et al., 2004). In the heart, VIP is expressed by nerve fibers, where it modulates heart rate, and coronary blood flow (Henning and Sawmiller, 2001). Chemicon's VPAC1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC1 interactions with VIP. The membrane preparations exhibit a Kd of 1.2 nM for [125I]-VIP. With 2.5 μg/well VPAC1 Membrane Prep and 0.4 nM [125I]-VIP, a greater than 5-fold signal-to-background ratio is obtained.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human VPAC1 / VIP1 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
应用说明	Radioligand binding assay, and GTPgammaS binding.

生物信息
浓缩	1 mL, 0.5 mg/mL per vial.
品种	Human
Entrez基因编号	NM_004624.2
Entrez基因汇总	This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase.
基因符号	RDC1 VIPR1 HVR1 PACAP-R-2 RCD1 VPCAP1R II FLJ41949 VIP-R-1 VAPC1 VPAC1 VIPR VIRG
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	VPAC1 / VIP1
靶子系列	VIP/PACAP
UniProt编号	P32241
UniProt汇总	FUNCTION: SwissProt: P32241 # This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38. SIZE: 457 amino acids; 51547 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: In lung, HT29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. SIMILARITY: SwissProt: P32241 ## Belongs to the G-protein coupled receptor 2 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C Radioligand: [125I] VIP (Perkin Elmer# NEX192) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled VIP at 0.4 nM

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of VPAC₁ membrane prep:

	10 µg/well	5 µg/well
Signal:Background	9.7	10.8
Specific Binding (cpm)	30299	31224

SPECIFICATIONS:
1 unit = 5 µg
Bmax: 19.65 pmol/mg
Kd: 1.2 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.