merck millipore,默克密理博,HTS040M,ChemiSCREEN™ Membrane Preparation Recombinant Human NOP Opioid Receptor

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay and GTPγS binding	Human	Chem-1	NOP / ORL1 / OP4

描述
产品目录编号	HTS040M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ Membrane Preparation Recombinant Human NOP Opioid Receptor
概述	Full-length human OPRL1 cDNA encoding NOP
背景信息	The NOP receptor (also known as ORL1) is related to the opioid receptor family of GPCRs but does not bind to classical opioids. An endogenous ligand for NOP has been characterized and termed orphanin FQ or nociceptin (OFQ/N), which in turn does not bind to other members of the opioid receptor family. NOP is expressed widely in the CNS, and binding of OFQ/N to NOP1 appears to function in nociception, locomotor activity, anxiety, reward, memory and tolerance to classical opioids (Mogil and Pasternak, 2001). Millipore's NOP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of NOP. The membrane preparations exhibit a Kd of 0.06 nM for [¹²⁵II]-Nociceptin. With 0.1 nM [¹²⁵I]-Nociceptin, 5μg/well NOP Membrane Prep typically yields greater than 10-fold signal-to-background ratio.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in 1 ml packaging buffer, rapidly frozen, and stored at -80°C

应用
应用	Human NOP / ORL1 / OP4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay and GTPγS binding

生物信息
品种	Human
Entrez基因编号	X72304
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	NOP / ORL1 / OP4
靶子系列	Opioid
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM Tris-HCl, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Tris-HCl, pH 7.4 filtered and stored at 4°C Radioligand: [¹²⁵I]-Nociceptin. (Perkin Elmer#:NEX-324 ) Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I labeled Nociceptin

产品使用声明

质量保证

Table 1. Signal:background and specific binding values obtained in a competition binding assay with NOP Receptor membrane prep.

	5 µg/well
Signal:Background	23.8
Specific Binding (cpm)	6656

SPECIFICATIONS: 1 unit = 5 µg
Bmax for [¹²⁵I]-Nociceptin binding: 1.1 pmol/mg protein
Kd for [¹²⁵I]-Nociceptin binding: ~0.06 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at –70°C up to the expiration date indicated on the product label. Do not freeze and thaw.

包装信息
数量	200 units

merck millipore,默克密理博,HTS040M,ChemiSCREEN™ Membrane Preparation Recombinant Human NOP Opioid Receptor

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech