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merck millipore,默克密理博,HTS025M,ChemiSCREEN™ Membrane Preparation Recombinant Human MCH1 Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human MCH1 Receptor
产品型号：HTS025M
Human MCHR1 / GPR24 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS025M,ChemiSCREEN™ Membrane Preparation Recombinant Human MCH1 Receptor
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重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 MCHR1 / GPR24 描述 产品目录编号 HTS025M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human MCH1 Receptor 概述 Full length human MCHR1 cDNA encoding MCH1 背景信息 Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992). When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001). A receptor for MCH, previously identified as an orphan receptor SLC-1 and renamed MCH1, has been identified in humans and rodents. Consistent with a role in hypothalmic control of feeding behavior, MCH1 is expressed in in the ventromedial and dorsomedial nuclei of the hypothalamus (Chambers et al., 1999). Genetic ablation and pharmacological antagonism of MCH1 in rodents results in resistance to obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002; Segal-Lieberman, et al., 2003; Shearman et al., 2003; Marsh et al., 2002; Chen et al., 2002). Thus, MCH1 is an attractive target for obesity. Millipore's MCH1membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of MCH1. The membrane preparations exhibit a Kd of 0.41 nM for [125I]-MCH. With 0.4 nM for [125I]-MCH, 5 μg/well MCH1 Membrane Prep yields greater than 8 fold signal-to-background ratio. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 8-fold signal:background with 125I-labeled MCH at 0.4 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human MCHR1 / GPR24 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_005297
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 MCHR1 / GPR24 靶子系列 Melanin-concentrating hormone 孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-MCH (Perkin Elmer NEX373)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C
产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with MCH1 membrane prep. 5 µg/well Signal:Background 13.9 Specific Binding (cpm) 7776
SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 1.9 pmol/mg
Kd: 0.41 nM
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,528875,PEG 1000 - CAS 25322-68-3 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,MABT409,Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody
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