merck millipore,默克密理博,HTS024RTA,Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells

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merck millipore,默克密理博,HTS024RTA,Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells
产品名称：Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells
产品型号：HTS024RTA
Human CRF2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

merck millipore,默克密理博,HTS024RTA,Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS024RTA,Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays; cAMP Assays	Human	Chem-1	CRF2

描述
产品目录编号	HTS024RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The corticotropin-releasing factor receptors, CRF1 and CRF2, are Gs-coupled GPCRs expressed in the brain, blood vessels and intestine that bind to several neuropeptides, including corticotropin-releasing factor (CRF) and urocortin, and the amphibian peptide sauvagine (Lovenberg et al., 1995; Bale and Vale, 2004). In addition, two peptides, urocortin II (Ucn II) and urocortin III (Ucn III), bind selectively and with high affinity to CRF2 (Lewis et al., 2001). The CRF peptides and their receptors play important roles in stress mediated by the hypothalamic-pituitary-adrenal axis in animal models, and possibly in depression and anxiety in humans, although the contributions of CRF1 and CRF2 appear to be distinct (Bale and Vale, 2004; Risbrough et al., 2004) In addition, CRF1 and CRF2 differentially alter feeding behavior, gastric motility and vascular tone (Zorrilla et al., 2004; Martinez et al., 2004; Wiley and Davenport, 2004). Millipore’s cloned human CRF2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CRF2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CRF2.

产品信息
部件	Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human CRF2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
应用说明	Calcium Flux Assays; cAMP Assays

生物信息
品种	Human
Entrez基因编号	NM_001883
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	CRF2
靶子系列	CRF Receptor
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

包装信息
数量	1 kit

merck millipore,默克密理博,HTS024RTA,Ready-to-Assay™ CRF2 Corticotropin Releasing Factor Receptor Frozen Cells

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