merck millipore,默克密理博,HTS023RTA,Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells

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merck millipore,默克密理博,HTS023RTA,Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells
产品名称：Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells
产品型号：HTS023RTA
Human CRF1 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

merck millipore,默克密理博,HTS023RTA,Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS023RTA,Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays; cAMP Assays	Human	Chem-1	CRF1

描述
产品目录编号	HTS023RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ CRF1 Corticotropin Releasing Factor Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. The CRF1 receptor is a Gs-coupled GPCR expressed in the brain and pituitary gland that binds to several neuropeptides, including corticotropin-releasing factor (CRF) and urocortin, and the amphibian peptide sauvagine (Chen et al., 1993; Dautzenberg and Hauger, 2002; Bale and Vale, 2004). CRF plays a predominant role in stress response mediated by the hypothalamic-pituitary-adrenal axis, and alterations in CRF and its receptors CRF1 and CRF2 appear to be linked to depression and anxiety (Holsboer, 1999; Bale and Vale, 2004). A number of small molecule antagonists of the CRF1 receptor have been characterized, including R121919, SC241, NBI27914, antalarmin, DMP-696, and CP 154,526. When delivered in animal models of psychiatric disorders, these antagonists display effectiveness in reducing stress-related behaviors (Kehne and De Lombaert, 2002). Millipore’s cloned human CRF1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CRF1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CRF1.

产品信息
部件	Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human CRF1 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
应用说明	Calcium Flux Assays; cAMP Assays

生物信息
品种	Human
Entrez基因编号	X72304
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	CRF1
靶子系列	CRF Receptor
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.