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应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay Human Chem-2 C3aR 描述 产品目录编号 HTS016M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ C3aR Membrane Preparation 概述 Full-length human C3aR cDNA 背景信息 C3a, along with C4a and C5a, is a 77 amino acid anaphylotoxin generated by proteolytic cleavage during activation of the complement pathway (Ember, 1997). The anaphylotoxins strongly promote inflammation by recruiting leukocytes, particularly basophils, eosinophils, neutrophils and monocyte (Martin, 1997). The proinflammatory properties of C3a are mediated by interaction between the peptide and a 7-TM G-protein coupled receptor, C3aR (Crass, 1996). Genetic and pharmacological inhibition of C3a/C3aR interaction indicates an important role for C3aR in allergic asthsma Humbles, 2000; Drouin, 2002). C3a/C3aR also enhances the effect of SDF-1 in promoting retention of haematopoietic stem/progenitor cells within the bone marrow (Rataiczak, 2004). Chemicon's C3aR membrane preps are an ideal tool for screening for compounds that disrupt binding of C3a to C3aR. The membranes have been shown to bind to 125I-labeled C3a with a Kd of 0.06 nM. 产品信息 格式 Membranes 演示 Membranes were adjusted to 0.5mg protein/mL in 50 mM Tris-HCL, pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 500 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled C3a (0.04 nM input concentration). 应用 应用 Human C3aR GPCR membrane preparation for Radioligand binding Assays. 应用说明 Radioligand binding assay 生物信息 浓缩 0.5mg/ml 品种 Human 品种反应性 Human Entrez基因编号 - NM_004054.2
基因符号 - AZ3B
- C3AR1
- C3a-R
- HNFAG09
- C3AR
- C3R1
GPCR类别 A 宿主细胞 Chem-2 蛋白靶 C3aR 靶子系列 Anaphylotoxin UniProt编号 - Q16581
UniProt汇总 FUNCTION: SwissProt: Q16581 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
SIZE: 482 amino acids; 53864 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
PTM: Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
SIMILARITY: SwissProt: Q16581 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 质量保证 Bmax for [125I]C3a binding: 1.9 pmol/mg protein
Kd for [125I] C3a binding: ~ 0.06 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 1 mL
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